Journal: Biochimica et biophysica acta

Article Title: Regulation of the release and function of tumor cell-derived soluble CD44.

doi: 10.1016/j.bbamcr.2005.02.006

Figure Lengend Snippet: Fig. 2. Molecular characterization of soluble CD44 from PMA-treated HTB58 cells. Cells were labeled with [35S]methionine (A, B) or [35S]sulfate (C, D) in the absence or presence of indicated cytokines for 48 h, washed once with culture media and then treated with 5107 M PMA or diluent control DMSO for 90 min (A, C) or 4 h (B, D). CD44 from conditioned media was then immunoprecipitated with anti-CD44 mAb followed by protein A/G plus agarose. Immune complexes were resolved on SDS-8% PAGE under non-reducing conditions followed by fluorography. Molecular weight markers in kDa are shown. OT=OSM+TGF-h1.

Article Snippet: HTB58 human lung squamous carcinoma cell line was obtained from the ATCC (Rockville, MD).

Techniques: Labeling, Control, Immunoprecipitation, Molecular Weight

Journal: Biochimica et biophysica acta

Article Title: Regulation of the release and function of tumor cell-derived soluble CD44.

doi: 10.1016/j.bbamcr.2005.02.006

Figure Lengend Snippet: Fig. 1. HTB58 cells shed CD44 in response to PMA. Cells were incubated for 48 h in MEM supplemented with 50 ng/ml OSM, 10 ng/ml TGF-h1 or both cytokines together (OT). Media were changed and cells were treated for 90 min with 5107 M PMA or diluent control DMSO. CD44 membrane levels, expressed as mean fluorescence intensity (MFI), were determined by flow cytometry using PE-conjugated anti-CD44 mAb. Paired results represent the cell surface expression of CD44 in the absence or presence of PMA. Data show one representative experiment out of four (A). The levels of CD44 in conditioned media collected at 90 min were quantified by ELISA (B and C). The data are shown as the mean of triplicate wells from five experimentsFS.D. (B). To block metalloprotei- nase activity, the cells were first pre-treated with 50 AM TAPI for 15 min at 37 8C and then treated with 50 AM TAPI simultaneously with PMA. The data are shown as the mean of duplicate wells from one representative experiment out of three (C). The difference between cells incubated in the absence or presence of PMA depicted in panel B was statistically significant as determined by Student’s t test ( P b0.05).

Article Snippet: HTB58 human lung squamous carcinoma cell line was obtained from the ATCC (Rockville, MD).

Techniques: Incubation, Control, Membrane, Fluorescence, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Blocking Assay, Activity Assay

Journal: Journal of Oncology

Article Title: GEMIN6 Overexpression Correlates with the Low Immune Cell Infiltration and Poor Prognosis in Lung Adenocarcinoma

doi: 10.1155/2022/1930604

Figure Lengend Snippet: GEMIN6 expression in LUAD and other types of human cancers from TCGA data. (a) GEMIN6 expression in different tumor types; (b) the expression of GEMIN6 in LUAD and its paired adjacent tissues; (c) the expression of GEMIN6 in LUAD and normal tissues; (d) the association of GEMIN6 expression and T stages in LUAD; (e) the association of GEMIN6 expression and pathologic stages in LUAD; (f) the association of GEMIN6 expression and N stages in LUAD; (g) relative expression of GEMIN6 in human bronchial epithelial cells (BEAS-2B) and LUAD cell lines (A549, H1299, SK-MES-1, PC-9, and NCI-H23); (h) receiver operating characteristic analysis (ROC) of GEMIN6 in LUAD.

Article Snippet: The A549, H1299, SK-MES-1, PC-9, and NCI-H23 LUAD cell lines and the normal human bronchial epithelial cell line BEAS-2B were purchased from the iCell Bioscience Inc. (China).

Techniques: Expressing

Journal: Oncotarget

Article Title: Dual-strand tumor-suppressor microRNA-145 ( miR-145-5p and miR-145-3p ) coordinately targeted MTDH in lung squamous cell carcinoma

doi: 10.18632/oncotarget.12290

Figure Lengend Snippet: A. Expression levels of miR-145-5p and miR-145-3p in lung SCC clinical specimens and cell lines (SK-MES-1 and EBC-1) were determined by qRT-PCR. Data were normalized to RNU48 expression. B. Correlation of the expression levels of miR-145-5p and miR-145-3p . C. Cell growth was determined by XTT assays 72 h after transfection with 10 nM miR-145-5p or miR-145-3p . * P < 0.05. D. Cell migration activity was determined by wound healing assays. * P < 0.001. E. Cell invasion activity was determined using Matrigel invasion assays. * P < 0.001.

Article Snippet: Two human lung SCC cell lines (SK-MES-1 and EBC-1) were obtained from Japanese Cancer Research Resources Bank (JCRB) and the American Type Culture Collection (Manassas, VA, USA), respectively.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Migration, Activity Assay

Journal: Oncotarget

Article Title: Dual-strand tumor-suppressor microRNA-145 ( miR-145-5p and miR-145-3p ) coordinately targeted MTDH in lung squamous cell carcinoma

doi: 10.18632/oncotarget.12290

Figure Lengend Snippet: A. Flow chart illustrates the strategy for analysis of miR-145-5p and miR-145-3p target genes in lung SCC cells. B. Identification of target genes of miR-145-5p or miR-145-3p . Expression levels of 7 mRNAs ( MTDH , EPN3 , TPD52 , CYP27B1 , LMAN1 , STAT1 and TXNDC12 ) were evaluated by qRT-PCR in EBC-1 cells 72 h after transfection with miR-145-5p or miR-145-3p . GUSB was used as an internal control. * P < 0.0001.

Article Snippet: Two human lung SCC cell lines (SK-MES-1 and EBC-1) were obtained from Japanese Cancer Research Resources Bank (JCRB) and the American Type Culture Collection (Manassas, VA, USA), respectively.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Control